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KMID : 0352419930120010045
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Keimyung Medical Journal
1993 Volume.12 No. 1 p.45 ~ p.54
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Effect of Parenteral Administration of Endotoxin on Changes of Serum and Hepatic Glutathione S-Transferase, Glutathione Peroxidase and Glutathione Reductase Activities in Rats
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곽춘식
문교철/김상철
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Abstract
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The activities of the cytosolic, microsomal and mitochondrial glutatione S-tranferase(GST) which are detoxifying enzymes in the liver were measured in order to evaluate the detoxifying ability of liver to endotoxin. The activities of glutathione
peroxidase(GSH-Px), glutathione reductase(GR) in the liver, GST and GR in the serum were also measured.
For administration of endotoxin, a dose of 5mg of endotoxin (lipopolysaccharide E, coli O26: B6, from Sigma chemical company, USA) per kg of body weight was administered through a right external jugular vein. Then the rats were killed after 3, 8
and 24
hours of injection with endotoxin to measure the activities of the above enzymes in serum and their liver.
The activities of the cytosolic and microsomal GST in the liver showed a significant decrease between 8 and 24 hours after endotoxin administration, but that of the mitochondria showed no signifcant changes throughout the experiment. The activity
of the
GR in the liver showed a significant decrease at 24 hours after endotoxin adiministration. The activity of the GSH-Px in the liver showed a significant decrease at 8 hours after endotoxin administration. Serum GST and GR activities showed a
signifcant
increase between 8 and 24 hours and between 3 and 24 hours after endotoxin administration respectively.
Acording to the results, cytosolic GST, microsomal GST and hepatic GR leak into the blood through the damaged membrane of hepatocyte. And it is suggested that the synthesis of the microsomal GST in the liver is decreased.
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